This horse deader than dead when ILMN's lawyer team destroyed the community college kid who worked for free this week...
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
@ singlemoleculer
Thanks for your advices. I checked my sequences but I am not sure about some points:
What does very rich of A's and T's mean? Do you have a percentage? The same with the low number of G's.
Of course I find CTAG's but where is the limit?
I also find many sequences with long stretches of polyA's polyT's? Is this also an indicator for bad sequencing?
Comment
-
The standard Helicos filtering software gets rid of these artifacts automatically. If you don't have access to the software, you can do manual filtering. Very rich in AT means A+T>90%. These are usually caused by problems in sample preparation. Any such sequences should be discarded unless you are working with a very AT-rich set of sequences like Plasmodium in which case you have to adjust the cutoff. For CTAG, you should look at the dimer frequencies. If CT+TA+AG+GC>70%, you should also discard. These sequences are usually caused when 2 DNAs are too close on the flow cell and the signals overlap. The filtering software actually has a more complex algorithm but this is good enough for most purposes.
Comment
Latest Articles
Collapse
-
by seqadmin
While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...-
Channel: Articles
06-06-2024, 07:15 AM -
-
by seqadmin
Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
Somatic Genomics
“We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...-
Channel: Articles
05-24-2024, 01:16 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 06:58 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:58 AM
|
||
Started by seqadmin, 06-06-2024, 08:18 AM
|
0 responses
20 views
0 likes
|
Last Post
by seqadmin
06-06-2024, 08:18 AM
|
||
Started by seqadmin, 06-06-2024, 08:04 AM
|
0 responses
18 views
0 likes
|
Last Post
by seqadmin
06-06-2024, 08:04 AM
|
||
Started by seqadmin, 06-03-2024, 06:55 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
06-03-2024, 06:55 AM
|
Comment