These are rough...and I can't find some of them on the Helicos web. Just thought I'd throw them up here if you don't want to go listen for yourself for 23 minutes.
50MB / hour out the door, 10-fold increase in 2 years to get to 500mb per day (>???) no addl capex for upgrades in perf.
10GB/run, 8 days
400 million reads (25-50 bp)
50 channels
<5% error rate, 0.5% substitution rate
error rate and length of read are independent
no GC bias (no sample prep...high call rate, no skewing (??)...basically due to no amp)
chemistry performance independent of template size (25bp - 8kb).
paired end read, sample prep free (on instrument)
per sample costs
$360 kit -->$381 w/external req'd reagents = 8 million reads (one lane)
EA instrument progressing well
2nd from major US cancer center, ship ~Oct 1st
13 in assembly phase, 8 can run samples
15-30 by mid 09
-----
business
10-fold increase in 2 years to get to 500mb per day (>???) no addl cap exp
single use kits
sequencer
linux cluster
$1.35 million list
kits $18,000 ($600k/year instrumnet, 900k within first year) (my calc: ~33 kits year = 10 day runs? = <1 GB/day).
$5M revenue stream 60-70% margin per instrument
Scalable production very hard (workflow), complex sample prep
tSMS: amp free solution
comparisons to leading NGS platform:
10Gb/run vs. 4.5Gb per run GAII as of 9/25 (didn't mention 8-10d vs. 3-4d)
100Gb/run with no hardware improvements
digital gene expression
candidate region resequencing
50 samples per run (vs 8)
6% sample premium (reagents beyond what's provided from HLCS) (265% with others), this is mostly all the library prep reagents. Not counting sample prep labor.
Milos presented "ton of data" on DGE and CRR in providence
10GB/run, 8 days
400 million reads (25-50 bp)
50 channels
<5% error rate, 0.5% substitution rate
error rate and length of read are independent
no GC bias (no sample prep...high call rate, no skewing (??)...basically due to no amp)
chemistry performance independent of template size (25bp - 8kb).
paired end read, sample prep free (on instrument)
per sample costs
$360 kit -->$381 w/external req'd reagents = 8 million reads (one lane)
EA instrument progressing well
2nd from major US cancer center, ship ~Oct 1st
13 in assembly phase, 8 can run samples
15-30 by mid 09
-----
business
10-fold increase in 2 years to get to 500mb per day (>???) no addl cap exp
single use kits
sequencer
linux cluster
$1.35 million list
kits $18,000 ($600k/year instrumnet, 900k within first year) (my calc: ~33 kits year = 10 day runs? = <1 GB/day).
$5M revenue stream 60-70% margin per instrument
Scalable production very hard (workflow), complex sample prep
tSMS: amp free solution
comparisons to leading NGS platform:
10Gb/run vs. 4.5Gb per run GAII as of 9/25 (didn't mention 8-10d vs. 3-4d)
100Gb/run with no hardware improvements
digital gene expression
candidate region resequencing
50 samples per run (vs 8)
6% sample premium (reagents beyond what's provided from HLCS) (265% with others), this is mostly all the library prep reagents. Not counting sample prep labor.
Milos presented "ton of data" on DGE and CRR in providence
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