Sample purification question

[glassfish]

Hi all,

I am going to drop off my first batch of DNA samples for sequencing next week. I plan to do a paired-end 101 sequencing of GAII. I sheared the DNA to 200bp by a Covarit. I plan to purify the DNA fragments with QIAquick gel extraction kit, but the technician from our core facility told me I don't need to purify them. I just want to check whether it is normal... Thanks a lot!

[jjenrette]

It is normal. You do not need to purify. The library prep protocol will contain many purification steps along the way.

[protist]

There is no need to gel purify - we do run the fragmented material through a Qiagen PCR column but that is only to concentrate the sample.