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Hi lilin001,
I don't know about the number of reads (it varies from user to user how much we load and how many clusters we get), but when we do our calculations based on the size of the smaller "real" peak and the results of the qPCR (which is KAPA for Illumina library quantification by the way), we tend to yield a predictable number of clusters time and again. We don't get more adapter reads than usual with these samples, nor do we get more noise.
I don't know about the number of reads (it varies from user to user how much we load and how many clusters we get), but when we do our calculations based on the size of the smaller "real" peak and the results of the qPCR (which is KAPA for Illumina library quantification by the way), we tend to yield a predictable number of clusters time and again. We don't get more adapter reads than usual with these samples, nor do we get more noise.
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