Streaks in flowcell images

[agent.hm]

Hi,

We were running our first run on a new GAIIx. the first base report was ok. however at the 2nd cycle.. weird things start to happen. i can see streaks occurring with different pattern ( see attached)

Anybody has any idea as to why this could have occured. As of now , we have stopped the run to troubleshoot.

[RPiddock]

My only theory is that the substrate is torn or is delaminating from the surface of the flow cell. This sometimes occurs when the cluster station or peltier heating block fails.

However, the images would probably look more like those below in later cycles.



Let me know if you figure out what went wrong.