I am having trouble size selecting on the TBE gels for the sm RNA preps? Any advice on what dyes and stains work best?
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staining TBE gel
We use SYBR for staining miRNA libraries post PCR. Our buffers gel loading buffers come from a kit. Resolution and separation of miRNAs products works well with SYBR. -
what percentage gel works best? did you use a TBE-Urea gel?Comment
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8% no ureaComment
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i was under the impression it is standard to use denaturing conditions when running RNA?Comment
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Hi
Not sure what are you asking for...isolating small RNAs or libraries made from miRNAs?
At least, I did it (small RNA isolation) with 15% denaturing (UreaGel from National Diagnostics) PAGE.
The library purification (eliminate any kind of primer dimer, adapters, etc) I used 10% non-denatuting PAGE.Comment
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