Do you think that there may be some non-random distribution of how RNA breaks in any sample determined by secondary structure? In other words, can you find any correlation between you bias that may be related on how RNA folds with itself?
-
-
@polivares: I have no idea. Seems like a possibility, but I'm not sure how to infer folding patterns, especially since we don't know how long the actual fragments are... only the first 25-30 bases or so... FYI: the fragmentation method was 'metal hydrolysis.'Comment
-
I would run different trials with tools like this with different window sizes helping me a bit with the known splicing sites :SComment
-
So, it seems pretty clear to me now that the towers are caused by PCR. The Illumina protocol (http://grcf.jhmi.edu/hts/protocols/m...04898_RevA.pdf) fragments the mRNA, and then amplifies it by PCR. So if a breakage happens to occur at a particular location, the PCR amplified dataset would have several copies of the same fragment. Do I understand this correctly?
Also, if this is the problem, then this presents a bit of a problem for quantifying AS events. Is there a way to PCR the mRNA before fragmentation instead?Last edited by behoward; 09-23-2009, 07:19 AM.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment