-I use 75X2 sequencing to get a good coverage.
-Meanwhile, I want a good coverage of different size: inset size from 40bp to 300bp.
My question is:
For the 75X2 sequencing, Can I sequence 40bp insert (40 bp DNA fragment within 2 ends' adapter sequencing)?
(I assume that for >75bp insert, sequencing should be no problem, and
I do not care that for 75x2 sequencing of 40bp, the sequencing read may contain adapter-sequence).
Thanks
Victor
-Meanwhile, I want a good coverage of different size: inset size from 40bp to 300bp.
My question is:
For the 75X2 sequencing, Can I sequence 40bp insert (40 bp DNA fragment within 2 ends' adapter sequencing)?
(I assume that for >75bp insert, sequencing should be no problem, and
I do not care that for 75x2 sequencing of 40bp, the sequencing read may contain adapter-sequence).
Thanks
Victor
Comment