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  • Malabady
    Member
    • Apr 2009
    • 12
    #1

    mRNAseq-data analysis

    Hello,
    I just got my Solexa data. I got the FastaQ files. Since this is my first time, I have some questions:
    1. What can be done to assure the quality of the sequences.?

    2. I obtained ~6 million reads per library, does this sound normal?

    3. How to convert Illumina quality scores to Phd scores?

    4. what is the best algorithm for de novo assembly.


    I appreciate any help
    Tags: None
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