Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Help!! need a copy of config.xml file from GAIIx run

    Hi,

    I need a copy of config.xml from a 100bp, single reads, GAIIx run (<runfolder>/Dana/Intensity/BaseCalls/config.xml)

    a lab in the campus acquired a GAIIx machine. After the first run, they found the RTA was configured improperly (long story). Anyway, now all intensity, bcl, stat, filter, etc. files are intact, but config.xml file is missing. So we could not do BCL to qseq conversion.

    But it could be re-constructed from a similar run. Well, it is the first run (other than a shorter phix test run earlier), they don't have a similar run. we modified a file from a 75bp reads run. But we run into errors later on.

    So if you happen to have a run from GAIIx for 100 base reads, single end, with 108 cycles (I think it is indexed), we would appreciate a copy of <runfolder>/Dana/Intensity/BaseCalls/config.xml

    thank you very much in advance!

    X.L.

  • #2
    Have you contacted illumina tech support ([email protected]) with this request? They are very helpful.
    Last edited by GenoMax; 11-04-2011, 06:18 AM.

    Comment


    • #3
      We did. And they told us how to modify the config.xml file. And that is how we modified the config.xml from a 75bp run.

      after posting this yesterday, I further examined the config.xml from that 75bp run. In the end I updated the RTA version number in the file, then added _pos.txt file location parameter in the setupBclToQseq.py command, and I was able to run the conversion.

      So problem might be solved. But I need to check the output files and make sure they are good.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      17 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      16 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      46 views
      0 likes
      Last Post seqadmin  
      Working...
      X