Originally posted by GenoMax
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IMHO this is not necessary; Lane X should look good, that's more important :-) We do this from time to time with chipseq data. -
If there was a control lane defined then there should be no need to go messing about with matrix or phasing settings (assuming the control lane looked OK).Originally posted by victorsor View Post
If you've still got no clusters then the only remaining remedy would be to run the bclToFastq with the --no-eamss option to see if the qualities temporarily dip and then recover.Comment
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Thank you all. We will try to do your suggestions when run finish. I hope that not all data go to waste...Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
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