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  • ein_io
    Member
    • Nov 2011
    • 14

    #1

    what specific sequence is used for annealing to flow cell?

    I am designing my own adapters
    and need to know which specific sequence is actually used for the annealing to the flow cell.

    I understand it is there inside the PE PCR primers sequence but I 'd like to specifically know which part of it, to properly judge what can I change/retain.

    Thanks million for any suggestions!!
  • huguesparri
    Member
    • May 2008
    • 97

    #2
    Hello,
    I think you can have a part of your answer here:
    http://seqanswers.com/forums/attachm...8&d=1239052303

    Comment

    • bbeitzel
      Member
      • Aug 2008
      • 50

      #3
      Here is a diagram that I drew up of the TruSeq process. The bridge PCR sequences (the oligos attached to the flow cell) are shown at the bottom of the diagram.
      Attached Files

      Comment

      • ein_io
        Member
        • Nov 2011
        • 14

        #4
        Thanks!

        Thank you very much huguesparri and bbeitzel!!
        That is what I was looking for

        Comment

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