Hi all,
as i am relatively new to the field i have a couple of questions:
1. Is it possible to do RNA-seq analysis when starting from cDNA? The lab guys dont have enough RNA so they wanna amplify first.
In my opinion that should not be a problem but i am not sure. On the web i found only one post where someone stated they started from cDNA.
2. Is it possible to do a Differential expression(DE) with Illumina PE data? For 'denovo' which i should also do is perfect but i am not sure about the DE.
Thank you for your time and any help is appreciated.
as i am relatively new to the field i have a couple of questions:
1. Is it possible to do RNA-seq analysis when starting from cDNA? The lab guys dont have enough RNA so they wanna amplify first.
In my opinion that should not be a problem but i am not sure. On the web i found only one post where someone stated they started from cDNA.
2. Is it possible to do a Differential expression(DE) with Illumina PE data? For 'denovo' which i should also do is perfect but i am not sure about the DE.
Thank you for your time and any help is appreciated.
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