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  • koakeson
    Junior Member
    • Nov 2008
    • 5
    #1

    Mate Pair protocol: hydro-shear vs. nebulization

    I am about to attempt my first Illumina mate pair library and I was wondering what the group felt was the best way to shear DNA, hydro-shear or nebulization? I have access to both and I am look to construct two libraries one with a 2kb insert and one with a 5kb insert.

    Thanks in advance for your advice.

    Kelly O.
    Tags: None
    • ECO
      --Site Admin--
      • Oct 2007
      • 1360
      #2
      In my experience, the hydroshear will make much tighter size distributions, however it is a bit more time consuming to use. Good luck.

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