Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Cluster Station Flow Problems

    We are having a terrible time with our newest lot of flow cells (42xxxAAXX). I am wondering if anyone else has had similar problems, and, hopefully, advice. I went from fairly reliably walking away from amp on the cluster station to having to watch the first cycles like a hawk and switching out 2-4 amp manifolds before one will pull evenly through. This is not a problem with the hyb manifolds and only seems to have started once we received the 42-series of flow cells. It is likely not a problem with our cluster stations as a manifold that fails on one cluster station fails on at least 2 of the 3 stations (two of the cluster stations have had practically everything replaced from the peltier heater, to the clips, to the pumps). I've tested new and old lots of manifolds and the problems seems to be irrespective of lot. The only thing that is new is the flow cells themselves. Has anyone had a good/bad experience with this lot of flow cells?

    Thanks!
    Last edited by SeaJane; 06-18-2009, 09:47 AM.

  • #2
    Hi, SeaJane:

    We got the same "42XXXAAXX" lot of FCs. We didn't experience the problem you described. Since all the manifolds were handmade, is that possilbe that you were just running into bad luck to have bad amplification manifold?

    We did have problem of very low cluster numbers with this lot of FCs. Just verified it wasn't the cluster station's problem this week. Just ordered a new cluster generation kit to verify if it's the problem with the reagent. Have you had such problem with your FC? Thanks a lot!

    Cheers!

    Comment


    • #3
      Hi,
      I experienced the same problem twice:
      - I've loaded a Flow Cell with 3 pM samples and I had less clusters in my run than usual.
      - I've tried another Flow Cell loaded with 5 pM samples and a 2pM PhiX: the amount of PhiX clusters was extremely low (like less than 500.000) and was surprisingly low for the samples (like 2.500.000 to 8.500.000 per sample) considering my starting concentration.

      Both Flow Cell's serial number were: 427XXAAXX

      I'll try another Flow Cell lot next week.
      We'll see...

      H.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Best Practices for Single-Cell Sequencing Analysis
        by seqadmin



        While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
        06-06-2024, 07:15 AM
      • seqadmin
        Latest Developments in Precision Medicine
        by seqadmin



        Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

        Somatic Genomics
        “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
        05-24-2024, 01:16 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 07:24 AM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 06-13-2024, 08:58 AM
      0 responses
      11 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 06-12-2024, 02:20 PM
      0 responses
      16 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 06-07-2024, 06:58 AM
      0 responses
      184 views
      0 likes
      Last Post seqadmin  
      Working...
      X