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  • Dr. Hybe
    Member
    • Jun 2009
    • 14

    #1

    Can a MiSeq be used as a Cbot?

    Would it be possible to perform cluster amplification and primer hyb. on the Miseq and then transfer the flowcell onto the HiSeq for sequencing? Just curious in the event your cBot were occupied. Thanks Dr. H
  • epistatic
    Senior Member
    • Mar 2009
    • 129

    #2
    The MiSeq can only cluster MiSeq flow cells. The flow cells are nothing alike in size nor connections to lane/lanes.

    The HiSeq 2500 is supposed to be able to cluster flow cells on the instrument, bypassing the cBot.

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    • pmiguel
      Senior Member
      • Aug 2008
      • 2328

      #3
      But only for "fast chemistry". The high output (old) chemistry will still require a cBot to cluster.

      --
      Phillip

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