Would it be possible to perform cluster amplification and primer hyb. on the Miseq and then transfer the flowcell onto the HiSeq for sequencing? Just curious in the event your cBot were occupied. Thanks Dr. H
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The MiSeq can only cluster MiSeq flow cells. The flow cells are nothing alike in size nor connections to lane/lanes.
The HiSeq 2500 is supposed to be able to cluster flow cells on the instrument, bypassing the cBot. -
But only for "fast chemistry". The high output (old) chemistry will still require a cBot to cluster.
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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