Has anyone been able to successfully amplify their genomic libraries in 12 cycles or less using the Illumina or NEB reagents for library prep and show successful sequencing?
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Have you seen this paper? With modifications to the ligated adapters you can avoid PCR completely.
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I do not have access to that article. would you be able to upload here or PM it to me?Comment
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You absolutely do not have a PM with the PDF attached...because that would be wrong.Comment
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thanks. got the paper
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You mean, on your own...right??Comment
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I am wondering why they 5´-phosphorylate both adapters in that paper.
The adapter they call A_adapter_b needs it for ligation to the DNA fragment, but the other one ?
Am I missing something for which it might be required ?
seqgirl, I used 10 cycles with 5 ug starting material for paired-end genomic DNA sequencing - no problem.
However, I want to give the PCR-free a try.
Thank you in advance if someone has a hint !Comment
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