TruSeq RNA library Agilent Bioanalyzer High Sensitivity DNA Chip. In green, the ds cDNA prior to ligation. In red after ligation but before any amplification. (Called "pre-amp final library" after this point.)
Notice that the pre-amp final library plot is trimodal. The first (left-most) peak would just be non-ligated cDNA. The middle peak looks reasonable for cDNA + both adapters. (150 bp insert + 120 bp of adapters).
What is that 3rd peak? Again, no amplification at this point. The samples were not heated enough to allow strand denaturation, at least not that I know of.
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Phillip
This is one of 12 samples. All show the same phenomenon.
Notice that the pre-amp final library plot is trimodal. The first (left-most) peak would just be non-ligated cDNA. The middle peak looks reasonable for cDNA + both adapters. (150 bp insert + 120 bp of adapters).
What is that 3rd peak? Again, no amplification at this point. The samples were not heated enough to allow strand denaturation, at least not that I know of.
--
Phillip
This is one of 12 samples. All show the same phenomenon.
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