Unknown reads quality format

[fuad193]

Hi

I have recently received a complete genome data in which reads quality look like this

93::499'2888521408):;%;*:7*81+3090.577774.6259;'82*=

what kind of quality is it and how to convert to standard fastq quality format ?

[Bukowski]

Hi

I have recently received a complete genome data in which reads quality look like this

93::499'2888521408):;%;*:7*81+3090.577774.6259;'82*=

what kind of quality is it and how to convert to standard fastq quality format ?

http://en.wikipedia.org/wiki/FASTQ_format

L - Illumina 1.8+ Phred+33, raw reads typically (0, 41)

[fuad193]

http://en.wikipedia.org/wiki/FASTQ_format

L - Illumina 1.8+ Phred+33, raw reads typically (0, 41)

So do I need to rescale or convert it before running BWA or Bowtie2 ?

[tonybolger]

So do I need to rescale or convert it before running BWA or Bowtie2 ?

There should be a flag to specify what the quality offset is (phred+33 or phred+64)