The old Illumina ligation conditions changed with the TruSeq kit. I had trouble swapping in TruSeq adapters for the old adapters using the old protocol, and I don't know why. Does anybody happen to know why, and by chance which ligase they're using now?
-
-
Hi jlove,
Could you be a bit more specific? The conditions changed from which kit to which other kit? (Cat numbers, if possible).
Very unlikely they switched ligases. For double-stranded DNA, T4 DNA ligase is nearly universally used.
If you mean you tried to swap in TruSeq Adapters for the adapters used in the Paired-End Sample Prep kit (PE ‐ 102‐ 1001/PE ‐ 102‐ 1002), then it may be that you would also need to use the TruSeq PCR primers for the enrichment PCR step.
--
Phillip
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment