Illumina Cancer Panel problems
We are getting either no or low yield after the PCR when running this assay. One thing we have noticed is that during the long annealing of the initial primers, there is substantial evaporation even using the recommended plates and seals. We switched to strip tubes which fixed this issue, however we continue to get very low yields whether on our samples or the positive controls. Has anyone else had issues like this with either the cancer panel or the custom amplicon panel? We have checked with Illumina technical service but have yet to figure out what is going on. Input would be appreciated.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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