Hi All,
We have a flow cell that we would like to re-analyze with pipeline, starting with image analysis. The reason is that this flow cell did not have a control lane, and all of the libraries had some sequence bias at the beginning, and this may have thrown off the base calling. We're planning to copy a previous run's control lane images into the image directory and then rerun the analysis, specifying the copied images as the control lane. Will this actually work? Has anyone ever tried anything like this before?
We have a flow cell that we would like to re-analyze with pipeline, starting with image analysis. The reason is that this flow cell did not have a control lane, and all of the libraries had some sequence bias at the beginning, and this may have thrown off the base calling. We're planning to copy a previous run's control lane images into the image directory and then rerun the analysis, specifying the copied images as the control lane. Will this actually work? Has anyone ever tried anything like this before?
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