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  • davejames
    Junior Member
    • Jun 2011
    • 1

    #1

    Thruplex Sample prep kit by Rubicon

    Thruplex sample prep kit by Rubicon? Anyone got experience of this kit? yields, diversity, coverage? cant see any literature out there from any users? appreciate any feedback
  • Goblin Wizard
    Junior Member
    • May 2009
    • 1

    #2
    Anyone got experience from this kit? I have tried to contact the company via their homepage (email submission form) but they are not responding. 48 reactions costs $7200, which is quite expensive for testing purposes. It was used in this paper:
    "Noninvasive whole-genome sequencing of a human fetus. Sci Transl Med. 2012 Jun 6;4(137):137ra76."

    Comment

    • mc1061dm1
      Junior Member
      • Oct 2012
      • 1

      #3
      I have the same question.

      Comment

      • jbechtol
        Junior Member
        • Sep 2012
        • 1

        #4
        Rubicon's ThruPLEX-FD kit

        Please visit our redesigned website at www.rubicongenomics.com for updated information and pricing on the ThruPLEX-FD kit. Kit prices have been adjusted to $4320.00 + shipping/handling per 48 rxn kit with volume discount pricing available.

        A 12 rxn size kit is also now available priced at $1140.00 + S&H.

        Contact us directly at [email protected] or call 734-677-4845 for further information.

        Thank you for your interest in Rubicon Genomics.

        Jennifer Bechtol
        Communications Manager
        Last edited by jbechtol; 07-02-2013, 11:43 AM. Reason: Wrong pre-fix for phone

        Comment

        • james hadfield
          Moderator
          Cambridge, UK
          Community Forum
          • Feb 2008
          • 224

          #5
          We used Thruplex in our recent Nature paper. http://dx.doi.org/doi:10.1038/nature12065, Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA. It was easy to run and gave great results from material many would not have considered it possible to sequence in this way.

          Comment

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