We are getting only 0.3-0.5 the coverage possible from our MiSeq when sequencing yeast genomic DNA using the Nextera kit for library generation. It seems to be due to tagmentation giving a larger than desired size distribution of fragments. Is yeast DNA a poorer Tn5 target than other DNA? We are using 50 ng DNA (picogreen assay) in the tagmentation reaction, as specified by the protocol. Would reducing the amount of DNA help?
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM