Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • export scaffold files from SAM/BAM alignment

    Using BWA, I have aligned ~10M MiSeq paired end reads to ~100 sequence contigs obtained from non-paired end 454 data. Most of the contigs remained intact after aligning the paired end data, but some were split due to zero coverage.

    Is it possible to export the FASTA sequences of the re-shuffled contigs directly from the BAM/SAM files? Furthermore, is there a way to also create the scaffold sequences from the BAM/SAM alignment based on mate pair information?

    Would you please let me know if there is a tool/pipeline or an alternative that can do the job?

    Thanks very much!

Latest Articles

Collapse

  • seqadmin
    Best Practices for Single-Cell Sequencing Analysis
    by seqadmin



    While isolating and preparing single cells for sequencing was historically the bottleneck, recent technological advancements have shifted the challenge to data analysis. This highlights the rapidly evolving nature of single-cell sequencing. The inherent complexity of single-cell analysis has intensified with the surge in data volume and the incorporation of diverse and more complex datasets. This article explores the challenges in analysis, examines common pitfalls, offers...
    Today, 07:15 AM
  • seqadmin
    Latest Developments in Precision Medicine
    by seqadmin



    Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.

    Somatic Genomics
    “We have such a tremendous amount of genetic diversity that exists within each of us, and not just between us as individuals,”...
    05-24-2024, 01:16 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 08:18 AM
0 responses
8 views
0 likes
Last Post seqadmin  
Started by seqadmin, Today, 08:04 AM
0 responses
10 views
0 likes
Last Post seqadmin  
Started by seqadmin, 06-03-2024, 06:55 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-30-2024, 03:16 PM
0 responses
27 views
0 likes
Last Post seqadmin  
Working...
X