I have about 100G metagenomic raw data sequenced by Illumina paired-end Hiseq2000. I meant to assemble the raw data by velvet. But there exists a big problem for me to assemble them at one time, while if I split the big file into several small parts and assemble them respectively, I maybe lost part of the data. So I am wondering If I could use several different K-mers to velvet the raw data separately and emerge all the results to assemble again?
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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