I have about 100G metagenomic raw data sequenced by Illumina paired-end Hiseq2000. I meant to assemble the raw data by velvet. But there exists a big problem for me to assemble them at one time, while if I split the big file into several small parts and assemble them respectively, I maybe lost part of the data. So I am wondering If I could use several different K-mers to velvet the raw data separately and emerge all the results to assemble again?
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by SEQadmin2
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...-
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by SEQadmin2
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...-
Channel: Articles
10-14-2026, 09:55 AM -
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