Library Quantification for pooling

[theward]

Hi,

I was wondering if anyone had any success with accurately quantifying libraries for pooling other than using the Qubit? I was wanting to make the process alot more high-throughput. Has anyone successfully used the Agilent Tapestion for this?

Thanks

[koadman]

The bead-based approach bundled in the Nextera XT kit seems to work fairly well for final library pooling. We've had some luck with picogreen assays on a tecan plate reader but it's not nearly as accurate as qpcr with the KAPA kits.

[pmiguel]

Hi,

I was wondering if anyone had any success with accurately quantifying libraries for pooling other than using the Qubit? I was wanting to make the process alot more high-throughput. Has anyone successfully used the Agilent Tapestion for this?

Thanks

Yes, KAPA qPCR is what you want. If you have a qPCR machine with sufficient capacity it can be very high-throughput.

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Phillip