Hey,
I plan to do a ChIP-Seq on the Illumina GA II. According to Illumina´s ChIP-Seq protocol, they use the primer pair PCR primer 1.1 and PCR primer 2.1 to amplifiy the library (ie adding the adapters).
But reading about the paired end read method, another primer pair was mentionend: the PCR primer PE 1.0 and 2.0
Does this mean, ChIP-Seq is not sequenced by paired end read, but only by single end read?
Or do I have to use the PCR primer PE 1.0 and 2.0 to do paired end read?
Looking forward to your answers!
I plan to do a ChIP-Seq on the Illumina GA II. According to Illumina´s ChIP-Seq protocol, they use the primer pair PCR primer 1.1 and PCR primer 2.1 to amplifiy the library (ie adding the adapters).
But reading about the paired end read method, another primer pair was mentionend: the PCR primer PE 1.0 and 2.0
Does this mean, ChIP-Seq is not sequenced by paired end read, but only by single end read?
Or do I have to use the PCR primer PE 1.0 and 2.0 to do paired end read?
Looking forward to your answers!
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