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  • kmcarr
    Senior Member
    • May 2008
    • 1181

    #1

    Control DNA sequencing primer

    I have looked over the excellent post above containing the sequences for all of the Solexa adapter/primer sequences (thanks ECO). However it did not specifically mention the sequencing primer for the phiX174 control DNA. Is the sequencing primer for the phiX control the same as the standard genomic sequencing primer?

    Thanks
  • ECO
    --Site Admin--
    • Oct 2007
    • 1360

    #2
    I'm not the person to ask...all the information I have at this time is in that Sticky post. I'm sure someone will chime in. I would expect them to be the same, but you never know.

    Comment

    • bkingham
      Member
      • May 2008
      • 11

      #3
      Yes, it is the same primer - also the same primer used for smRNA sequencing, would be helpful if they let us know if it were the same for all kits

      Comment

      • elg
        Junior Member
        • Mar 2008
        • 7

        #4
        No, for DGE kits the primer used is a different one

        Comment

        • bkingham
          Member
          • May 2008
          • 11

          #5
          Interesting.....I looked at the website that shows the primer sequences, they appear to be different for all the kits - but I have used the same primer for PhiX, smRNA, and genomic sequencing. I wonder if the ligated pieces are the same and they just choose different parts to design primers for different kits?

          Comment

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