I am starting with 500ng DNA [using cell lines] for DNA library prep using SureSelect XT protocol. I am getting very low DNA conc after amplification ,using 10 cycles. It is not enough for me to hybridise for Hiseq.
What could be inhibiting my DNA conc....changed all reagents,using brand new kit ....same kit being used by others in the lab and is working...
Any advice appreciated.
Thanks
Dummyseq
What could be inhibiting my DNA conc....changed all reagents,using brand new kit ....same kit being used by others in the lab and is working...
Any advice appreciated.
Thanks
Dummyseq
Comment