I have a hiseq run and in Lanes 2, 4, and 6 I have Ns at base 18 in all sequences. Similar library types are run in other lanes and do not have this problem. Anyone have any reasons why this could happen?
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Originally posted by SLB View PostI have a hiseq run and in Lanes 2, 4, and 6 I have Ns at base 18 in all sequences. Similar library types are run in other lanes and do not have this problem. Anyone have any reasons why this could happen?
Contact Illumina Tech Support (or have your sequencing core do so) and have them look at the run data (stored in the InterOp folder).
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