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  • 100bp paired-end RNA-seq library

    Hi folks,

    Does anyone have experience making libraries for 100bp paired-end sequencing? What is the optimal insert size? How to generate fragments suitable for this purpose?Thanks.

  • #2
    These two protocols are very good:

    High-Throughput Illumina Strand-Specific RNA Sequencing Library Preparation.
    Cold Spring Harb Protoc. 2011;2011(8)
    Authors: Zhong S, Joung JG, Zheng Y, Chen YR, Liu B, Shao Y, Xiang JZ, Fei Z, Giovannoni JJ


    A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq
    Lin Wang, Yaqing Si, Lauren K. Dedow, et al. 2011 PLoS ONE 6(10)

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