Hey,
I want to have the primers used on TruSeq kit make on a different company from Illumina.
I have the following sequences:
PCR Primer 1.0
5 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA 3
PCR Primer 2.0
5 CAAGCAGAAGACGGCATACGAGAT
It is supposed to have phosphorothioate bonds between the two last (3') nucleotides? I checked the primers for the RRBS protocol ( nature) and the phosphorothioate bonds are between a C and T. So, these primers (1.0 and 2.0) should have this modification? If even they doesn't have CT in their 3'end?
Someone has tried that before?
Thx
I want to have the primers used on TruSeq kit make on a different company from Illumina.
I have the following sequences:
PCR Primer 1.0
5 AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGA 3
PCR Primer 2.0
5 CAAGCAGAAGACGGCATACGAGAT
It is supposed to have phosphorothioate bonds between the two last (3') nucleotides? I checked the primers for the RRBS protocol ( nature) and the phosphorothioate bonds are between a C and T. So, these primers (1.0 and 2.0) should have this modification? If even they doesn't have CT in their 3'end?
Someone has tried that before?
Thx
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