Dear all,
this is my first post so be kind. I have a stupid question but after days of reading Illumina protocols my brain has ceased to function.
I have 24 samples which I wish to run through the MiSeq, I have a Truseq kit with 12 indices and I am using the Low Throughput protocol which suggests a single index, how do I index 24 samples?
I have previously used the Nextera kit with i5 and i7 indices which made sense but I'm not sure I get how a single index works.
Thanks in advance.
this is my first post so be kind. I have a stupid question but after days of reading Illumina protocols my brain has ceased to function.
I have 24 samples which I wish to run through the MiSeq, I have a Truseq kit with 12 indices and I am using the Low Throughput protocol which suggests a single index, how do I index 24 samples?
I have previously used the Nextera kit with i5 and i7 indices which made sense but I'm not sure I get how a single index works.
Thanks in advance.
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