Hi,
In both of MiSeq manual and Custom Amplicon Prep. Guide, it is said that maximum 96 samples can be read per run. It is seen that number of i5 and i7 primers (w/ indices) limits the number of samples.
There are 8 different i5 primers and 12 different i7 primers and combination of these create tags for 96 samples. Is it possible to design additional i5 or i7 primers (w/ custom indices) to increase sample number that can be read per run?
Thank you,
In both of MiSeq manual and Custom Amplicon Prep. Guide, it is said that maximum 96 samples can be read per run. It is seen that number of i5 and i7 primers (w/ indices) limits the number of samples.
There are 8 different i5 primers and 12 different i7 primers and combination of these create tags for 96 samples. Is it possible to design additional i5 or i7 primers (w/ custom indices) to increase sample number that can be read per run?
Thank you,
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