Hi everyone!!!
I am working with Sure select capture , and the miseq in a DNAseq experiment.
This is my first time that I use the paired end read at 150bp , and I have a problem at the end of the reads, I have a bias in the last bases. I remove all reads with Q<30 , remove adaptors with cutadapt and trimomatic, but the bias is not resolved.
The mean of the insert size is 198bp , and the adaptors are the tipical for illumina.
The size mean of the library is 350bp before sequecing.
this happens someone???
Thanks in advance.
I am working with Sure select capture , and the miseq in a DNAseq experiment.
This is my first time that I use the paired end read at 150bp , and I have a problem at the end of the reads, I have a bias in the last bases. I remove all reads with Q<30 , remove adaptors with cutadapt and trimomatic, but the bias is not resolved.
The mean of the insert size is 198bp , and the adaptors are the tipical for illumina.
The size mean of the library is 350bp before sequecing.
this happens someone???
Thanks in advance.
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