Front manifold leaked

[btran33]

Hi All:
I'm running a small sequencing center with 4 GAIIx; for the last few months we had experienced a high number of front manifold leaking, which resulted in a number of lost runs as well as down time. Illumina kept insisted that this is not a well known issue, but I don't believe that; therefore I would like to see if any one has the same problem and kindly let me know.
thank you
btran33

[ScottC]

We're yet to have any leaks in the actual GAIIx.

We did have leaks on our cluster station manifolds for many months before they replaced parts of it.

[anders]

We have also have a leaking front manifold on our GaIIx. The Illumina technician attributed the problem to corrosion and we will get it replaced. We were instructed not to leave the flowcell in place between runs in the future as that could contribute to the problem [During our initial training we were instructed that we should leave the flowcell in place between runs].

The first two runs after the upgrade to GaIIx went well. In the third run, we did not see any problems when installing the flowcell, but we saw some bubbles in one of the lanes when looking at the images afterwards. Before the fourth run, we ran some tests and since we could not get the manifold to stop leaking we called for the Illumina technician.

[btran33]

Thank you Scott and Anders for quick reply. I'm still working with Illumina to correct the problem, but so far it's seem like we got a bad lot of front GA manifolds.