Help to plan experiment

[Nullie]

Hello everyone,

I am a biology student and I need to check the microbial population in the intestine before and after taking a certain medication, to see if the drug affects the microbe abundance in the intestine... I need to plan this experiment using NGS and I am LOST!
please can someone help me to plan this experiment...? what NGS technology should I use after extraction of DNA/RNA samples?

Thank You!

[microgirl123]

Have you looked into 16s rDNA sequencing? It may not be the best solution, but there are lots of papers out there using this method.

[rhinoceros]

1. Think very hard what biological questions you want to address
2. Find out how much funding you have
3. Read a ton of papers
4. Make an informed decision