[sequencer13]

Yes, I'm afraid you're probably right. We'll do a quick phiX run and then try again with a more dilute sample.

It is odd though, as we frequently use cluster densities in that ball park and have never seen anything like this before.

[GenoMax]

It is odd though, as we frequently use cluster densities in that ball park and have never seen anything like this before.

You must be making very good quality libraries.

I guess your luck finally ran out

[sequencer13]

Just a quick update / resolution:

The phiX test run was fine, and we ran the libraries again at a lower concentration; the cluster density only dropped about 100K so the cluster density from the previous run must have hit some sort of cap.

In short - too concentrated libraries. Oops >.<

Thanks again for all the comments.