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  • illuminaGA
    Member
    • Dec 2012
    • 71
    #1

    What is "oxo" in the PE flow-cell oligo ‘D’?

    Dear All, I have a question about the "oxo" in the PE flow-cell oligo ‘D’.

    5’-PS-TTTTTTTTTTAATGATACGGCGACCACCGAGAUCTACAC-3’ (U = 2-deoxyuridine) PE flow-cell oligo ‘C’
    5’-PS-TTTTTTTTTTCAAGCAGAAGACGGCATACGAGoxoAT-3’ PE flow-cell oligo ‘D’

    What is it? What is the function of oxo?
    What is the function of U in oligo C?

    Thank you so much.
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