where to purchase reversible terminators

[ein_io]

Hello dear sequencing gurus

I was wondering whether anybody knows where and if I could purchase separately the reversible terminators used in Illumina kits.

We would like to fiddle a bit with sequencing by synthesis protocol.

would deeply appreciate any advise

Thanks!!

[GW_OK]

I would be incredibly surprised if that is not a proprietary reagent. You could check and see where they got it in their original patents, though, if it wasn't custom made for them...

[ein_io]

Thanks!

well the formula and the synthesis protocol is fully disclosed in the papers and it does not seem to be too hard to make. So I was surprised to find nobody sells them.

[GW_OK]

The market share is probably worth less than a possible IP suit.

[krobison]

Contact Azco Biotech; I think they're now out of that business, but they may still sell them for the Polonator/Mini.

There have been multiple published reversible terminator chemistries, but perhaps as GW_OK suggested they are all locked up by patents licensed to the players in this field (QIAGEN, Illumina, Helicos, LaserGen, etc).

[ein_io]

krobinson – huge thanks for the info!!
will check it out asap