I'm planning on sending out for sequencing soon using a MiSeq PE 250. I was wondering if its possible to use two Read 1 primers at once? I have a 16s sample that contains the PCR from two different primers (a general Bac and specific). Will running the sample with a Gen Bac Read 1, Specific Read 1, and their reverse Read 2 work? Or do I need to split them up into two seperate runs? I'm afraid it may cause interference.
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Originally posted by FNB View PostI'm planning on sending out for sequencing soon using a MiSeq PE 250. I was wondering if its possible to use two Read 1 primers at once? I have a 16s sample that contains the PCR from two different primers (a general Bac and specific). Will running the sample with a Gen Bac Read 1, Specific Read 1, and their reverse Read 2 work? Or do I need to split them up into two seperate runs? I'm afraid it may cause interference.
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