I'm planning on sending out for sequencing soon using a MiSeq PE 250. I was wondering if its possible to use two Read 1 primers at once? I have a 16s sample that contains the PCR from two different primers (a general Bac and specific). Will running the sample with a Gen Bac Read 1, Specific Read 1, and their reverse Read 2 work? Or do I need to split them up into two seperate runs? I'm afraid it may cause interference.
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Originally posted by FNB View PostI'm planning on sending out for sequencing soon using a MiSeq PE 250. I was wondering if its possible to use two Read 1 primers at once? I have a 16s sample that contains the PCR from two different primers (a general Bac and specific). Will running the sample with a Gen Bac Read 1, Specific Read 1, and their reverse Read 2 work? Or do I need to split them up into two seperate runs? I'm afraid it may cause interference.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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