Hi, I am planning to sequence
a series of human tumors by the nextera rapid capture expanded exome kit.
Using the HiSeq1000, to obtain 100x coverage, the "coverage calculator for Illumina Sequencin systems" recommends loading pools of 6 samples/lane (with 200 cycles).
But I was told that it is less expensive to make 12-samples enrichment pools and load them twice (12samples/lane, 2 lanes).
Can anybody confirm this or give me other suggestions?
a series of human tumors by the nextera rapid capture expanded exome kit.
Using the HiSeq1000, to obtain 100x coverage, the "coverage calculator for Illumina Sequencin systems" recommends loading pools of 6 samples/lane (with 200 cycles).
But I was told that it is less expensive to make 12-samples enrichment pools and load them twice (12samples/lane, 2 lanes).
Can anybody confirm this or give me other suggestions?
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