Gel Breaker Tubes for Illumina Truseq smallRNA

[Constanza Muñoz]

Hi, Does anyone know if there is a substitute of the gel breaker tubes used in the Illumina protocol "Truseq smallRNA" ? Because I work in a sequencing lab in Chile and we´re having troubles to find the gel breaker tubes required in the protocol.
Thanks !

[cascoamarillo]

Hi. When I was working on small RNAs (not Truseq protocol), to extract RNAs from acrylamide gels I just crushed the gel band inside the 1.5ml tube and elute the RNA in 500ul of 0.3M NaCl (shake or quake overnight). Then, move the supernatant to a new tube and precipitate RNA and keep going to the next step.