Hi,
I am sequencing libraries on MiSeq (V2 and V3) and facing constant problems with my i7 reads. At the end of my read1, the quality drops and remains low during my i7 sequencing (16 cycles).
However, the quality of my read2 (which initiates from the same read2 sequence-Nextera Read2 sequencing primer- as for i7) is perfect.
My question is: how can you get good quality for read2 but bad quality for i7, given that the sequencing primer is the same? and is anyone else facing the same problem?
Thanks!
Best,
Walid
I am sequencing libraries on MiSeq (V2 and V3) and facing constant problems with my i7 reads. At the end of my read1, the quality drops and remains low during my i7 sequencing (16 cycles).
However, the quality of my read2 (which initiates from the same read2 sequence-Nextera Read2 sequencing primer- as for i7) is perfect.
My question is: how can you get good quality for read2 but bad quality for i7, given that the sequencing primer is the same? and is anyone else facing the same problem?
Thanks!
Best,
Walid
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