Hello NGS community. I have a question that I'd greatly appreciate your ideas on. We recently conducted an RNAseq analysis for a samples with a specific focus on small RNA to long non-coding RNA. To achieve this, we employed the P1 2x300 cycle sequencing approach to cover the entire range of RNAs, from approximately 20bp to 700bp because the Tapestation electropherogram showed peak from 175 to 700 bp. However, we have observed a decrease in run quality, and a significant portion of our reads had no overlapping sequences between Read 1 and Read 2.
Can someone explain to me why we are experiencing poor run quality and the absence of overlapping nucleotides?
Thank you!
Can someone explain to me why we are experiencing poor run quality and the absence of overlapping nucleotides?
Thank you!
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