Hi everyone, I have recently sequenced 1 lane of NovaSeq X Plus 10B with a sequencing provider. The library that was sequenced is a multiplex WGS library.
I received the result and was informed that I have high undetermined reads (approximately 56%) in my library.
They have also sent me the possible indexes for the undetermined reads and I was unable to find any of the sequences in my index list.
The index that I have used for the sequencing was used in the past as well and nothing of sort has happened before.
This is the %Pass filter vs %Occupied plot. The %Pass filter is in the 40-50% range, is there something wrong about this run?
Thank you for your kind help in advance!
I received the result and was informed that I have high undetermined reads (approximately 56%) in my library.
They have also sent me the possible indexes for the undetermined reads and I was unable to find any of the sequences in my index list.
The index that I have used for the sequencing was used in the past as well and nothing of sort has happened before.
This is the %Pass filter vs %Occupied plot. The %Pass filter is in the 40-50% range, is there something wrong about this run?
Thank you for your kind help in advance!
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