Hello
We have a problem with Illumina Miseq v2 Micro (no clustering or only 250K) 3 runs the last one in a different MiSeq (another lab). The library is Paragon genomics amplicon based (CFTR and BRCA) that we have loaded before with no problem. Part of the present library was loaded on a nano v2 with perfect results, so it is not the library, and we have also changed NaOH so it is not the denaturation. The MiSeq passed all tests so it is not the sequencer.
Has anyone been having problems with Illumina reagents or flow cell Micro V2?
Please help as the costs are enormous and the distributor is trying NOT to replace anything....
Thanks
Chris
We have a problem with Illumina Miseq v2 Micro (no clustering or only 250K) 3 runs the last one in a different MiSeq (another lab). The library is Paragon genomics amplicon based (CFTR and BRCA) that we have loaded before with no problem. Part of the present library was loaded on a nano v2 with perfect results, so it is not the library, and we have also changed NaOH so it is not the denaturation. The MiSeq passed all tests so it is not the sequencer.
Has anyone been having problems with Illumina reagents or flow cell Micro V2?
Please help as the costs are enormous and the distributor is trying NOT to replace anything....
Thanks
Chris
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