In the Paired-End SamplPrep_Guide_1005063_B protocol, there is a QC step to check the recovered DNA to ensure the presence of at least 0.5ug of fragmented DNA before performing end repair. When I follow old protocol, there is no such step, I usually check DNA using nanodrop after nebulization, never recover over 500ng of fragment DNA, but my library works fine so far. I am curios if any one has problem making libraries with less than 0.5ug of fragment DNA? thanks!
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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