In the Paired-End SamplPrep_Guide_1005063_B protocol, there is a QC step to check the recovered DNA to ensure the presence of at least 0.5ug of fragmented DNA before performing end repair. When I follow old protocol, there is no such step, I usually check DNA using nanodrop after nebulization, never recover over 500ng of fragment DNA, but my library works fine so far. I am curios if any one has problem making libraries with less than 0.5ug of fragment DNA? thanks!
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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